Dr Nadanai Laohakunakorn, originally from Bangkok, obtained his BA/MSci in Natural Sciences (Physics) from Trinity College, Cambridge in 2010. He remained in Cambridge to carry out his PhD under the supervision of Prof Ulrich Keyser, where he studied nanopores and single-molecule biophysics. In particular, his work focused on electrically-driven fluid flows generated within the confined geometries of nanopores, and he developed techniques to measure and characterise these effects using optical tweezers. After defending his thesis in 2015, he moved to Lausanne where he worked with Prof Sebastian Maerkl at EPFL, on the new and growing field of cell-free synthetic biology. His work there focused on combining microfluidic devices with cell-free gene expression systems for high-throughput and rapid prototyping of genetic parts and circuits. In 2019 he received a Chancellor's Fellowship in Biotechnology at the University of Edinburgh, enabling him to establish a lab for quantitative engineering of cell-free systems. In 2021 he received a UKRI Future Leaders Fellowship.

NL

Current members


Dr Sahan Liyanagedera is working in collaboration with the Edinburgh Genome Foundry on developing high-throughput methods to screen the PURE system against putative components which are hypothesised to enhance either its yield or its function. Originally from Sri Lanka, he grew up in London and went on to study Biomedical Sciences at the Peninsula School of Medicine, University of Plymouth and graduated in 2015. Excited by the rapid advances in Biotechnology, he pursued a Masters in Research in Synthetic Biology at UCL under the supervision of Dr Darren Nesbeth. Here he developed a de-novo Transketolase and Transaminase fusion enzyme that could be utilised for the one-pot synthesis of chiral amino-alcohols in the industrially relevant host Pichia pastoris. In 2017 he secured a 4-year EPSRC-funded PhD position in the lab of Dr Vishwesh Kulkarni at the University of Warwick. During this time, he developed the TXTL-based SpyPhage Platform that enables the rapid cell-free production and post-translational modification of bacteriophages for phage therapy. In his spare time, he enjoys playing football and cricket with friends and long bicycle rides on gravel tracks in the countryside.

SL

Dr Antonios Bougas is working on a collaborative project between the Laohakunakorn group and the Burgess group at the EdinOmics facility. He is investigating the role of metabolites derived from energy regeneration pathways and their impact on protein synthesis which is used to power cell-free systems. Originally from Corinthia, he graduated from the Department of Biology, University of Patras, Greece. Subsequently, he secured a PhD fellowship through the competitive call from the Greek State Scholarships Foundation and carried on his postgraduate studies at the Department of Biochemistry, School of Medicine, Patras. During that time, he focused on the study of both eukaryotic and bacterial ribosomes, in the process of evaluating novel protein synthesis inhibitors. Specifically, he investigated the kinetics and context-specificity of antibiotics targeting the bacterial ribosome, as well as their effect on the emerging translatome using high-throughput techniques. During his leisure time he enjoys playing basketball with friends and practising amateur photography as an excuse to travel to new places.

AB

Sarah Paterson is a PhD student funded by the Leverhulme Trust working on cell-free protein synthesis using high throughput microfluidics. She holds a Master's in pharmacy from the University of Strathclyde as well as an MSc in Biomedical Engineering. Sarah gained experience in cell culture working within the start-up company “ScreenIn3D” using microfluidic platforms to create 3D tumour microenvironments for drug screening assays. She is interested in applying engineering principles to the design and construction of biological systems. As a keen runner you’ll find Sarah running around muddy fields on weekends competing in cross country races.

SP

Surendra Yadav is a Darwin Trust doctoral student who's working to construct synthetic metabolism to efficiently power PURE cell-free systems. Before joining the University of Edinburgh for his Ph.D, he completed his Masters from the Indian Institute of Science Education and Research, Mohali, India. During his Master's thesis, he worked under the supervision of Prof. Anand K. Bachhawat to investigate the role of 6-phosphogluconate dehydrogenase in cellular metabolism of Saccharomyces cerevisiae. Being a curious undergrad student he tried to dive and gain experience in different fields of biology by doing various internships and summer research projects. In his undergraduate studies he worked with Dr. Lolitika Mandal at IISER Mohali to understand hematopoiesis, and utilized the UAS-GAL4 bipartite system for the development of various uncommon traits in Drosophila melanogaster such as ectopic eyes. He was intrigued by the field of synthetic biology and its useful applications while he was undergoing his Masters thesis, and decided to pursue his Ph.D in cell-free synthetic biology. Surendra is a cricket enthusiast, and also likes to spend his free-time playing guitar and sipping tea.

SY

Alex Perkins is a BBSRC doctoral student working on the scaling up of industrial cell-free protein expression in partnership with Fujifilm Diosynth Biotechnologies. Joining the group from Imperial College, he completed an MRes in Synthetic Biology under the watchful eyes of Drs Polizzi and Ceroni where he worked on optimising quorum-sensing devices using directed evolution and investigating metabolic burden via RNA-seq analysis. In the private sector, he spent a stint working under Dr Martin on neonatal stem-cell storage and differentiation at Cells4Life and collaborated with OpenCell to develop a cheap, opensource, automated, high-throughput Covid-19 RT-qPCR testing system. As a fresh-faced undergraduate, he obtained a Biomed BSc from UCL where he focused on molecular mechanisms of HepC-induced cellular changes and the lasting effects of Ebola virus during convalescence. Originally hailing from the Sussex countryside, he can be found cooking, coding, and writing in the third person. He is interested in how modern computing and statistical methods can be applied to Bio Big Data to find better solutions in bioengineering applications.

AP

Michael Stam is a PhD student funded by the CDT in Biomedical AI. He holds a BSc in Mathematics from the University of Edinburgh and works on using machine/deep learning for de novo protein design with cell-free systems, in collaboration with Chris Wood's and Diego Oyarzún's groups.

MS

Paula Paredes Vergara: I’m an undergraduate student currently studying for an MSci in biochemistry at the University of Glasgow. During June-August 2022 I’ll be working in Dr Laohakunakorn’s group to design and test a strategy for assembling linear DNA templates for use in cell-free protein synthesis (CFPS) reactions. I’ll be using Golden Gate assembly to create these templates and explore this technique for the design of circularized DNA templates as well. With this project I hope to establish protocols and demonstrate that this technique can be very useful and less time consuming than the current cloning methods used in CFPS. My aim during my time here is to learn new molecular techniques and explore the field of synthetic biology which already greatly interests me.

PPV

Alumni

  • Tim Bosse, Hons student (Edinburgh)
  • Yan Ting Hee, Hons student (Edinburgh)
  • Dominik Kopčak, Undergraduate student (Edinburgh)
  • Katie Bexley, Integrative Cell Mechanisms rotation student (Edinburgh)
  • Meijie Liu, MSc student (Edinburgh)
  • Bastien Mollet, VRes exchange student (ENS Lyon)
  • Sasirasa Pratumsuwan, Hons student (Edinburgh)
  • Yehezkiel Victorio, MSc student (Edinburgh)
  • Ying Yang, MSc and IGEM student (Edinburgh)
  • Yutong Ke, MSc student (Edinburgh)
  • Maria Fernandez, Hons student (Edinburgh)
  • Baird McIlwraith, Hons student (Edinburgh)
lab
lab
lab
lab
lab
lab
lab
lab
lab

lab
lab
lab
lab
lab
lab
lab